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Pranam D. Chatterjee


Assistant Professor of Biomedical Engineering

We develop diverse algorithmic modalities to discover, characterize, and engineer novel proteins for applications in genome editing, targeted protein modulation, and reproductive bioengineering.

Our long-term goals are two-fold:

  1. Enable the de novo design of protein effectors for any biological purpose
  2. Leverage our platform technologies to prolong and promote reproductive longevity and equality

Appointments and Affiliations

  • Assistant Professor of Biomedical Engineering
  • Assistant Professor of Computer Science

Contact Information

  • Office Location: 101 Science Drive, CIEMAS 2353A, Durham, NC 27705
  • Email Address:
  • Websites:


  • Ph.D. Massachusetts Institute of Technology, 2020

Research Interests

Integration of computational and experimental methodologies to design novel proteins for applications in genome editing, targeted protein modulation, and reproductive bioengineering

Courses Taught

  • BME 390L: Special Topics with a Lab
  • BME 493: Projects in Biomedical Engineering (GE)
  • BME 494: Projects in Biomedical Engineering (GE)
  • BME 590: Special Topics in Biomedical Engineering
  • BME 791: Graduate Independent Study
  • EGR 393: Research Projects in Engineering

In the News

Representative Publications

  • Liang, S-Q; Liu, P; Ponnienselvan, K; Suresh, S; Chen, Z; Kramme, C; Chatterjee, P; Zhu, LJ; Sontheimer, EJ; Xue, W; Wolfe, SA, Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag., Nature Methods (2023) [10.1038/s41592-023-01859-2] [abs].
  • Koseki, S; Hong, L; Yudistyra, V; Stan, T; Tysinger, E; Silverstein, R; Kramme, C; Amrani, N; Savic, N; Pacesa, M; Rodriguez, TS; Ponnapati, M; Jacobson, J; Church, G; Truant, R; Jinek, M; Kleinstiver, B; Sontheimer, E; Chatterjee, P, PAM-Flexible Genome Editing with an Engineered Chimeric Cas9., Res Sq (2023) [10.21203/] [abs].
  • Pierson Smela, MD; Kramme, CC; Fortuna, PRJ; Adams, JL; Su, R; Dong, E; Kobayashi, M; Brixi, G; Kavirayuni, VS; Tysinger, E; Kohman, RE; Shioda, T; Chatterjee, P; Church, GM, Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression., Elife, vol 12 (2023) [10.7554/elife.83291] [abs].
  • Kobayashi, M; Kobayashi, M; Odajima, J; Shioda, K; Hwang, YS; Sasaki, K; Chatterjee, P; Kramme, C; Kohman, RE; Church, GM; Loehr, AR; Weiss, RS; Jüppner, H; Gell, JJ; Lau, CC; Shioda, T, Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers, Stem Cell Reports, vol 17 no. 3 (2022), pp. 507-521 [10.1016/j.stemcr.2022.01.012] [abs].
  • Palepu, K; Ponnapati, M; Bhat, S; Tysinger, E; Stan, T; Brixi, G; Koseki, SRT; Chatterjee, P, Design of Peptide-Based Protein Degraders via Contrastive Deep Learning (2022) [10.1101/2022.05.23.493169] [abs].