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Professor of Biomedical Engineering
Bursac's research interests include pluripotent stem cell therapies for heart and muscle disease. Cardiac and skeletal muscle tissue engineering. Cardiac electrophysiology and arrhythmias. Genetic modifications of stem and somatic cells. Micropatterning of proteins and hydrogels. Organ-on-chip technologies.
The focus of my research is application of stem cells and tissue engineering methodologies in experimental in vitro studies and cell and tissue replacement therapies. Micropatterning of extracellular matrix proteins or protein hydrogels and engineering of synthetic scaffolds are used to build stem cell-derived cardiac and skeletal muscle tissues that replicate the structure-function relationships present in healthy and diseased muscle. These systems are used to separate and systematically study the roles of structural and genetic factors that contribute cardiac and skeletal muscle function and disease at multiple organizational levels (from single cell to 3-dimensional tissue). Optical recordings with voltage and calcium sensitive dyes in synthetic tissues allow us to analyze and optimize normal electrical function as well as study complicated spatio-temporal changes in electrical activity encountered in cardiac arrhythmias and fibrillation. Contractile force measurements allow us to explore factors that would optimize mechanical function of engineered tissues. Examples of the current research projects include: 1) design of co-cultures made of cardiac and different types of stem cells to model and study cell and tissue therapies for cardiac infarction and arrhythmias, 2) local and global gene manipulation in cultures of cardiac and other cell types, 3) engineering of vascularized cardiac and skeletal muscle tissue constructs with controllable structure and function, 4) implantation of stem cell-derived cardiac tissue patches in animal models of cardiac infarction, and 5) design of synthetic excitable tissues for experimental studies and novel cell therapies.
Appointments and Affiliations
- Professor of Biomedical Engineering
- Associate Professor in Medicine
- Professor in Cell Biology
- Member of the Duke Cancer Institute
- Co-Director of the Regeneration Next Initiative
- Office Location: CIEMAS 1141, Durham, NC 27708
- Office Phone: (919) 660-5510
- Ph.D. Boston University, 2000
- B.S.E. University of Belgrade, 1994
Bursac's research interests include embryonic and adult stem cell therapies for heart and muscle disease. Cardiac and skeletal muscle tissue engineering. Cardiac electrophysiology and arrhythmias. Genetic modifications of stem and somatic cells. Micropatterning of proteins and hydrogels.
- BME 301L: Bioelectricity (AC or GE)
- BME 394: Projects in Biomedical Engineering (GE)
- BME 493: Projects in Biomedical Engineering (GE)
- BME 494: Projects in Biomedical Engineering (GE)
- BME 507: Cardiovascular System Engineering, Disease and Therapy (GE, BB, EL)
- BME 578: Quantitative Cell and Tissue Engineering (GE, BB, MC)
- BME 791: Graduate Independent Study
- BME 792: Continuation of Graduate Independent Study
- EGR 393: Research Projects in Engineering
- NEUROSCI 301L: Bioelectricity (AC or GE)
In the News
- Building a Better Brain (Aug 6, 2018 | Duke Medicine Alumni Magazine )
- Engineers Grow Functioning Human Muscle from Skin Cells (Jan 9, 2018 | Pratt School of Engineering )
- Beating Heart Patch is Large Enough to Repair the Human Heart (Nov 28, 2017 | Pratt School of Engineering )
- Bacterial Genes Boost Current in Human Cells (Oct 18, 2016)
- Tissue-Patching a Broken Heart (Oct 6, 2016)
- Nerd Watch video: Duke researchers work to grow custom muscles (Mar 24, 2015 | NBC News )
- First Contracting Human Muscle Grown in Lab (Jan 13, 2015)
- Self-healing muscles (May 23, 2014 | UNC-TV’s "North Carolina Now" )
- Scientists progress in quest to grow muscle tissue (Apr 8, 2014 | The Wall Street Journal )
- Scientists create the first lab-grown muscle that's 'as strong as the real thing’ (Apr 2, 2014 | The Independent )
- Scientists grow muscles in the lab that can heal themselves (Apr 1, 2014 | NBC News )
- Self-Healing Engineered Muscle Grown in the Laboratory (Apr 1, 2014)
- Self-healing muscle grown in the lab (Apr 1, 2014 | BBC News )
- Gokhale, TA; Asfour, H; Verma, S; Bursac, N; Henriquez, CS, Microheterogeneity-induced conduction slowing and wavefront collisions govern macroscopic conduction behavior: A computational and experimental study., Plos Computational Biology, vol 14 no. 7 (2018) [10.1371/journal.pcbi.1006276] [abs].
- Koeberl, DD; Case, LE; Smith, EC; Walters, C; Han, S-O; Li, Y; Chen, W; Hornik, CP; Huffman, KM; Kraus, WE; Thurberg, BL; Corcoran, DL; Bali, D; Bursac, N; Kishnani, PS, Correction of Biochemical Abnormalities and Improved Muscle Function in a Phase I/II Clinical Trial of Clenbuterol in Pompe Disease., Molecular Therapy (2018) [10.1016/j.ymthe.2018.06.023] [abs].
- Nguyen, HX; Kirkton, RD; Bursac, N, Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies., Nature Protocols, vol 13 no. 5 (2018), pp. 927-945 [10.1038/nprot.2018.016] [abs].
- Khodabukus, A; Prabhu, N; Wang, J; Bursac, N, In Vitro Tissue-Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease., Advanced Healthcare Materials (2018) [10.1002/adhm.201701498] [abs].
- Jackman, CP; Ganapathi, AM; Asfour, H; Qian, Y; Allen, BW; Li, Y; Bursac, N, Engineered cardiac tissue patch maintains structural and electrical properties after epicardial implantation., Biomaterials, vol 159 (2018), pp. 48-58 [10.1016/j.biomaterials.2018.01.002] [abs].