FIP Seminar: Super Resolution, Light Sheet, and Smart Microscopy

Conventional microscopes are too slow, too phototoxic, or lack the spatial resolution to capture many complex cellular processes. We are developing novel light sheet fluorescence microscopes for rapid three-dimensional imaging with minimal phototoxicity. Current work incorporates three focus areas – applying adaptive optics to see clearly deep into living organisms, super-resolution single molecule and structured illumination microscopy to enhance spatial resolution, and novel computational algorithms to pull insight from vast quantities of dynamic three-dimensional data. These advances will improve current observations of living systems and enable fundamentally new investigations in cell biology. Relevant publications: Shi et al. 2024, Shi et al. 2022, Legant et al. 2016, Chen et al. 2014