Bioinspired Engineering of Human Pluripotent Stem Cell Fate Choices
Thursday, December 4, 2014
4:30 pm - 5:30 pm
Fitzpatrick Center Schiciano Auditorium Side A
Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold tremendous promise in tissue engineering and regenerative medicine applications because of their unique combination of two properties, pluripotency and an extremely high proliferative capacity. Theoretically, almost unlimited supplies of cells and tissues could be generated from a single clonal source if we can regulate PSC growth and differentiation. Several critical factors regulate whether a PSC chooses to self-renew or differentiate. Soluble signals bind receptors and stimulate chemical pathways that lead to global changes in gene transcription and cell differentiation state. This talk will present data illustrating differentiation of pluripotent stem cells to blood-brain barrier microvascular endothelial cells (BMECs) by co-culture of endothelial progenitors with developing neural cell types. These BMECs are currently being used to construct in vitro models of the blood-brain barrier to predict drug passage to the brain.