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FIP Seminar: Co-hosted with Duke OSA/SPIE Student Optical Chapter "Quantitative Phase Imaging in Biomedicine"

Jan 23

Wednesday, January 23, 2019

12:00 pm - 1:00 pm
Fitzpatrick Center Schiciano Auditorium Side B, room 1466


Dr. Gabriel Popescu, Professor of Electrical and Computer Engineering & Bioengineering, University of Illinois at Urbana-Champaign

Light scattering limits the quality of optical imaging of unlabeled specimens: too little scattering and the sample is transparent, exhibiting low contrast, and too much scattering washes the structure information altogether. As a result, current instruments, target specifically either the thin (low-scattering) specimens or the optically thick (multiply scattering) samples. In 2011 we developed spatial light interference microscopy (SLIM) as a highsensitivity, high-resolution quantitative phase imaging method, which open new applications for studying structure and dynamics. Color SLIM (cSLIM) is a recent development that allows the phase imaging of stained tissue slices. Using specimens prepared under the standard protocols in pathology, cSLIM yields simultaneously the typical image that the pathologist is accustomed to (e.g., H&E, immunochemical stains, etc.) and a quantitative phase image, which provides new information, currently not available in bright field images (e.g., collagen fiber orientation). However, SLIM works best for thin specimens, such as single cell layers and tissue slices. To expand this type of imaging to thick, multiply scattering media, we developed gradient light interference microcopy (GLIM). GLIM exploits the principle of low-coherence interferometry to extract phase information, which in turn yields strong, intrinsic contrast of transparent samples, such as single cells.

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