MEMS Seminar: Ultrasonic 3D Cell Culture for Solid Tumor Micro-Engineering
Monday, November 20, 2017
12:00 pm - 1:00 pm
Hudson Hall 125
Professor Martin Viklund
In this talk I will present our recent research activities in solid tumor micro-engineering based on ultrasound-supported three-dimensional (3D) cell culture in a multi-well microplate, developed in my lab at the KTH-Royal Institute of Technology, Stockholm, Sweden. The method is gentle and can be used for producing various tissue-mimicking 3D structures in parallel. It is based on ultrasonic-standing-wave particle manipulation inside hundred micro-wells in the microplate. We analyze and optimize the driving parameters of the ultrasound transducer attached to the microplate, and we demonstrate the production of various micro-engineered models of solid tumors. We have primarily used cells from HepG2 hepatocellular carcinoma and A498 renal carcinoma cell lines as building blocks in the tumor models, but we have also tested the method with fibroblasts, thyroid and melanoma cells. Martin Wiklund is a professor in applied physics at KTH-Royal Institute of Technology, Stockholm, Sweden, but currently on sabbatical leave at Amherst College, USA. After undergraduate studies at LTH-Lund Institute of Technology, Lund, Sweden (M.Sc. in Engineering Physics, 1999; Profile: Biomedical Optics), Wiklund received a Ph.D. in Physics in 2004 from KTH-Royal Institute of Technology.