FIP Seminar: Probing neural circuits with shaped light
Wednesday, October 4, 2017
12:00 pm - 1:00 pm
Fitzpatrick Center Schiciano Auditorium Side B
Dr. Na Ji, Associate Professor, Department of Physics and Department of Molecular and Cell Biology, University of California at Berkeley
To understand computation in the brain, one needs to understand the input-output relationships for neural circuits and the anatomical and functional relationships between individual neurons therein. Optical microscopy has emerged as an ideal tool in this quest, as it is capable of recording the activity of neurons distributed over millimeter dimensions with sub-micron spatial resolution. I will describe how we use concepts in astronomy and optics to develop next-generation microscopy methods for imaging neural circuits at higher resolution, greater depth, and faster speed. By shaping the wavefront of the light, we have achieved synapse-level spatial resolution through the entire depth of primary visual cortex, optimized microendoscopes for imaging deeply buried nuclei, and developed a video-rate (30 Hz) volumetric imaging method. We apply these methods to understanding neural circuits, using the mouse primary visual cortex as our model system. Na Ji studied chemistry and physics as an undergraduate in the University of Science and Technology of China and later a graduate student at University of California Berkeley. In 2006, she moved to Janelia Research Campus, Howard Hughes Medical Institute and worked with Eric Betzig on improving the speed and resolution of in vivo brain imaging. She started her own group in Janelia in 2011, where, in addition to imaging technology development, her lab apply the resulting techniques to outstanding problems in neurobiology.