FIP Seminar: Functional optical imaging, from single molecules to human eyes

Sep 6

Wednesday, September 6, 2017

12:00 pm - 1:00 pm
Fitzpatrick Center Schiciano Auditorium Side B


Dr. Hao F. Zhang, Professor, Biomedical Engineering Department, Northwestern University

My lab focuses on two optical imaging technologies, optical coherence tomography (OCT) and photon localization microscopy, to fill the gaps in both clinical diagnoses and fundamental biomedical investigations. To enable OCT to extract physiological and pathological information beyond high-quality anatomical imaging, we developed visible-light OCT or vis-OCT. Operating within the visible-light spectral range, vis-OCT has demonstrated great potential in ultra-high resolution imaging, angiogram, oxygen metabolic imaging, and ultrastructural pathological sensing. We are applying vis-OCT to investigate several blinding diseases (diabetic retinopathy, retinal vein occultation, macular degeneration, and glaucoma) and ischemic strokes. In our super-resolution imaging work, we developed spectroscopic photon localization microscopy (SPLM). Traditional photon localization microscopy analyses the spatial distributions of photons emitted stochastically by individual molecules to reconstruct super-resolution optical images. SPLM further captures the inherent spectroscopic signatures of these photons. Through molecular discrimination and regression, SPLM can reach the spatial resolution of 10¿nm or greater without significantly increasing the total number of image frames. Using SPLM, we demonstrated simultaneous multi-molecular super-resolution imaging, where the number of fluorescence labels can have largely overlapping emission spectra with only minute differences.

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